Journal: Journal of fluorescence
Article Title: Studies of DNA Aptamer OliGreen and PicoGreen Fluorescence Interactions in Buffer and Serum
doi: 10.1007/s10895-016-1840-1
Figure Lengend Snippet: (A) Unstained 12% polyacrylamide SDS gel showing lane 1; unstained Bio-Rad Amplisize® DNA ladder, lane 2; 30 ng of Eco 3R aptamer in rat serum with OG, lane 3; 30 ng of ERK aptamer in rat serum with OG, lane 4; 30 ng of Eco 3R aptamer in rat serum with PG, lane 5; 30 ng of ERK aptamer in rat serum with PG during UV illumination. (B) the same gel as in panel A following EtBr staining. (C) Lane 1; Bio-Rad Amplisize® DNA ladder, lane 2; 5 μg of stock Eco 3R aptamer in TE buffer and lane 3; 5 μg of stock ERK aptamer in TE buffer electrophoresed in a 1% agarose EtBr stained gel. DNA ladder standard lengths are shown in base pairs (bp).
Article Snippet: Interestingly, the aptamers in also appear to form higher molecular weight concatamers or partial hybrids with themselves which may effect OG or PG fluorescence intensities. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 10 caption a7 (A) Unstained 12% polyacrylamide SDS gel showing lane 1; unstained Bio-Rad Amplisize ® DNA ladder, lane 2; 30 ng of Eco 3R aptamer in rat serum with OG, lane 3; 30 ng of ERK aptamer in rat serum with OG, lane 4; 30 ng of Eco 3R aptamer in rat serum with PG, lane 5; 30 ng of ERK aptamer in rat serum with PG during UV illumination. (B) the same gel as in panel A following EtBr staining. (C) Lane 1; Bio-Rad Amplisize ® DNA ladder, lane 2; 5 μg of stock Eco 3R aptamer in TE buffer and lane 3; 5 μg of stock ERK aptamer in TE buffer electrophoresed in a 1% agarose EtBr stained gel.
Techniques: SDS-Gel, Staining
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